Virus-Derived Vectors for the Expression of Multiple Proteins in Plants

Pooja Saxena; Eva C Thuenemann; Frank Sainsbury; George P Lomonossoff

doi:10.1007/978-1-4939-3289-4_3

Virus-Derived Vectors for the Expression of Multiple Proteins in Plants

Methods Mol Biol. 2016:1385:39-54. doi: 10.1007/978-1-4939-3289-4_3.

Authors

Pooja Saxena¹, Eva C Thuenemann², Frank Sainsbury³, George P Lomonossoff⁴

Affiliations

¹ Department of Chemistry, Indiana University, Bloomington, IN, USA.
² Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich, UK.
³ Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, St Lucia, QLD, Australia.
⁴ Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich, UK. george.lomonossoff@jic.ac.uk.

PMID: 26614280
DOI: 10.1007/978-1-4939-3289-4_3

Abstract

This chapter constitutes a practical guide to using the "pEAQ" vector series for transient or stable expression of one or more protein(s) in Nicotiana benthamiana plants. The pEAQ vectors are a series of small binary vectors designed for controlled expression of multiple proteins in plants. To achieve high levels of expression, an expression system based on translational enhancement by the untranslated regions of RNA-2 from cowpea mosaic virus (CPMV), named CPMV-HT, is used. The expression vector pEAQ-HT combines the user-friendly pEAQ plasmid with CPMV-HT to provide a system for high-level expression of proteins in plants.

Keywords: Agro-infiltration; CPMV-HT; Transient expression; Viral vectors; pEAQ.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Comovirus / genetics*
Genetic Engineering / methods*
Genetic Vectors*
Nicotiana / genetics*
Plants, Genetically Modified*
Plasmids
Recombinant Proteins*

Substances

Recombinant Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding