Abstract
Posttranslational modification (PTM) of proteins and peptides is important for diverse biological processes in plants and animals. The paucity of heterologous expression systems for PTMs and the technical challenges associated with chemical synthesis of these modified proteins has limited detailed molecular characterization and therapeutic applications. Here we describe an optimized system for expression of tyrosine-sulfated proteins in Escherichia coli and its application in a bio-based crop protection strategy in rice.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Agriculture / methods
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Crop Protection / methods
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Crops, Agricultural
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Escherichia coli / genetics*
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Green Fluorescent Proteins / genetics
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Oryza / genetics
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Oryza / microbiology*
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Peptides / chemistry
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Plasmids / metabolism
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Protein Processing, Post-Translational
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Recombinant Proteins / biosynthesis*
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Recombinant Proteins / chemistry
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Synthetic Biology / methods*
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Tyrosine / analogs & derivatives*
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Tyrosine / chemistry
Substances
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Peptides
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Recombinant Proteins
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Green Fluorescent Proteins
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tyrosine O-sulfate
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Tyrosine