Application of RNA-Seq Technology in Cancer Chemoprevention

Methods Mol Biol. 2016:1379:31-43. doi: 10.1007/978-1-4939-3191-0_4.

Abstract

RNA-sequencing is a revolutionary tool to follow differential expression after treatment with cancer chemopreventive agents. It allows a real genome-wide screening independent of prior assumptions and is well suited for analyzing coding but also long noncoding RNAs. It still consents the discovery of new genes and isoforms and increased our knowledge of antisense and other noncoding RNAs in a tremendous manner. Moreover, it permits to detect low-abundance and biologically critical isoforms and reveals genetic variants and gene fusions in one single assay. Here, we provide a detailed protocol for stranded RNA-sequencing.

Keywords: Chemoprevention; Deep-sequencing; Long noncoding RNA; Novel transcript and isoform discovery; RNA-Seq; Stranded RNA-Seq; Transcriptional signature; Transcriptome profiling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chemoprevention*
  • Humans
  • Neoplasms / genetics*
  • Neoplasms / prevention & control*
  • Polymerase Chain Reaction
  • Quality Control
  • RNA / chemistry
  • RNA / genetics*
  • RNA / isolation & purification
  • Reproducibility of Results
  • Reverse Transcription
  • Ribosomes / genetics
  • Sequence Analysis, RNA / methods*

Substances

  • RNA