Characterization of a gamma-butyrolactone synthetase gene homologue (stcA) involved in bafilomycin production and aerial mycelium formation in Streptomyces sp. SBI034

Appl Microbiol Biotechnol. 2016 Mar;100(6):2749-60. doi: 10.1007/s00253-015-7142-8. Epub 2015 Nov 25.

Abstract

Streptomyces SBI034 produces several bafilomycin derivatives. Its afsA homologue (stcA) and putative γ-butyrolactone receptor gene (stcB) were cloned. Construction of a stcA disruptant (stcA gene knockout) resulted in complete abolishment of all bafilomycin production. Electron microscopic analysis showed a defect of aerial mycelium formation and sporulation in the stcA disruptant. Restoration of all phenotypic defects and bafilomycin production was observed in a stcA complemented strain. Addition of exogenous γ-butyrolactone (GBL) extracted from the culture broth of the wild-type strain could stimulate the aerial mycelium and spore formation of the stcA disruptant. These results suggest that stcA plays a role in GBL-mediated regulation of bafilomycin biosynthesis and morphological development in Streptomyces strain SBI034.

Keywords: A-factor; Bafilomycin; Streptomyces; γ-Butyrolactone regulatory system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 4-Butyrolactone / metabolism*
  • Enzyme Inhibitors / metabolism*
  • Gene Knockout Techniques
  • Genetic Complementation Test
  • Ligases / genetics*
  • Ligases / metabolism*
  • Macrolides / metabolism*
  • Spores, Bacterial / growth & development
  • Streptomyces / enzymology*
  • Streptomyces / growth & development
  • Streptomyces / metabolism*

Substances

  • Enzyme Inhibitors
  • Macrolides
  • bafilomycin A
  • Ligases
  • 4-Butyrolactone