Outline of a bacterial filter-based assay to detect beta-lactamases

B B Wintermans; C M J E Vandenbroucke-Grauls

doi:10.1016/j.mimet.2015.11.013

Outline of a bacterial filter-based assay to detect beta-lactamases

J Microbiol Methods. 2016 Jan:120:29-33. doi: 10.1016/j.mimet.2015.11.013. Epub 2015 Nov 18.

Authors

B B Wintermans¹, C M J E Vandenbroucke-Grauls¹

Affiliation

¹ Department of Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands.

PMID: 26602625
DOI: 10.1016/j.mimet.2015.11.013

Abstract

We describe a new phenotypic test to detect beta-lactamases. This assay is based on diffusion of beta-lactam/beta-lactamase through a bacterial filter. Beta-lactam hydrolysis on (the other side of) the filter leads to a change in antibiotic susceptibility, which can be measured by disc diffusion tests. We illustrate its ease of use to detect beta-lactamases of different classes.

Keywords: Beta-lactamase; Carbapenemase; ESBL; Phenotypic method; Resistance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacteria / drug effects
Bacteria / enzymology*
Bacteria / metabolism
Bacterial Proteins / analysis
Bacterial Proteins / chemistry*
Bacteriological Techniques*
Disk Diffusion Antimicrobial Tests
Microbial Sensitivity Tests / methods
Phenotype
beta-Lactam Resistance
beta-Lactamases / analysis
beta-Lactamases / chemistry*
beta-Lactams / metabolism

Substances

Anti-Bacterial Agents
Bacterial Proteins
beta-Lactams
beta-Lactamases
carbapenemase