Double-Layer Magnetic Nanoparticle-Embedded Silica Particles for Efficient Bio-Separation

San Kyeong; Cheolhwan Jeong; Homan Kang; Hong-Jun Cho; Sung-Jun Park; Jin-Kyoung Yang; Sehoon Kim; Hyung-Mo Kim; Bong-Hyun Jun; Yoon-Sik Lee

doi:10.1371/journal.pone.0143727

Double-Layer Magnetic Nanoparticle-Embedded Silica Particles for Efficient Bio-Separation

PLoS One. 2015 Nov 24;10(11):e0143727. doi: 10.1371/journal.pone.0143727. eCollection 2015.

Authors

Affiliations

¹ School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea.
² Nano Systems Institute and Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul, Korea.
³ Center for Theragnosis, Korea Institute of Science and Technology, Seoul, Korea.
⁴ Department of Bioscience and Biotechnology, Konkuk University, Seoul, Korea.

Abstract

Superparamagnetic Fe3O4 nanoparticles (NPs) based nanomaterials have been exploited in various biotechnology fields including biomolecule separation. However, slow accumulation of Fe3O4 NPs by magnets may limit broad applications of Fe3O4 NP-based nanomaterials. In this study, we report fabrication of Fe3O4 NPs double-layered silica nanoparticles (DL MNPs) with a silica core and highly packed Fe3O4 NPs layers. The DL MNPs had a superparamagnetic property and efficient accumulation kinetics under an external magnetic field. Moreover, the magnetic field-exposed DL MNPs show quantitative accumulation, whereas Fe3O4 NPs single-layered silica nanoparticles (SL MNPs) and silica-coated Fe3O4 NPs produced a saturated plateau under full recovery of the NPs. DL MNPs are promising nanomaterials with great potential to separate and analyze biomolecules.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ferrosoferric Oxide / chemistry*
Kinetics
Magnetite Nanoparticles / chemistry*
Nanotechnology / methods*
Silicon Dioxide / chemistry*

Substances

Magnetite Nanoparticles
Silicon Dioxide
Ferrosoferric Oxide

Grants and funding

This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Science, ICT & Future Planning(2014-A002-0065). Consulting service from Microbial Carbohydrate Resource Bank (MCRB) is gratefully acknowledged.