Therapy of Chronic Cardiosclerosis in WAG Rats Using Cultures of Cardiovascular Cells Enriched with Cardiac Stem Cell

E V Chepeleva; S V Pavlova; A A Malakhova; E A Milevskaya; Ya L Rusakova; N A Podkhvatilina; D S Sergeevichev; E A Pokushalov; A M Karaskov; G T Sukhikh; S M Zakiyan

doi:10.1007/s10517-015-3119-y

Therapy of Chronic Cardiosclerosis in WAG Rats Using Cultures of Cardiovascular Cells Enriched with Cardiac Stem Cell

Bull Exp Biol Med. 2015 Nov;160(1):165-73. doi: 10.1007/s10517-015-3119-y. Epub 2015 Nov 24.

Authors

E V Chepeleva^{1

2

3}, S V Pavlova^{1

2

3}, A A Malakhova^{1

2

3}, E A Milevskaya^{3

4}, Ya L Rusakova³, N A Podkhvatilina³, D S Sergeevichev³, E A Pokushalov³, A M Karaskov³, G T Sukhikh⁵, S M Zakiyan^{6

7

8

9}

Affiliations

¹ Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Moscow, Russia.
² Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Moscow, Russia.
³ E. N. Meshalkin Research Institute of Circulatory Pathology, Ministry of Health of the Russian Federation, Moscow, Russia.
⁴ Novosibirsk National Research State University, Novosibirsk, Russia.
⁵ V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
⁶ Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Moscow, Russia. zakian@bionet.nsc.ru.
⁷ Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Moscow, Russia. zakian@bionet.nsc.ru.
⁸ E. N. Meshalkin Research Institute of Circulatory Pathology, Ministry of Health of the Russian Federation, Moscow, Russia. zakian@bionet.nsc.ru.
⁹ Novosibirsk National Research State University, Novosibirsk, Russia. zakian@bionet.nsc.ru.

PMID: 26597688
DOI: 10.1007/s10517-015-3119-y

Abstract

We developed a protocol for preparing cardiac cell culture from rat heart enriched with regional stem cells based on clonogenic properties and proliferation in culture in a medium with low serum content. Experiments on WAG rats with experimental ischemic myocardial damage showed that implantation of autologous regional stem cells into the left ventricle reduced the volume of cicatricial tissue, promoted angiogenesis in the damaged zone, and prevented the risk of heart failure development.

Keywords: cardiac stem cells; cell therapy; myocardial infarction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens, Differentiation / analysis
Antigens, Differentiation / genetics
Cell Differentiation
Cell Division
Cells, Cultured
Culture Media / pharmacology
Disease Models, Animal
Fetal Blood
Genes, Reporter
Heart Atria / cytology
Heart Failure / prevention & control
Heart Ventricles
Lectins / metabolism
Lipoproteins, LDL / metabolism
Male
Multipotent Stem Cells / cytology
Multipotent Stem Cells / drug effects
Myocardial Ischemia / surgery*
Myocardium / pathology
Myocytes, Cardiac / cytology
Myocytes, Smooth Muscle / cytology
Myofibroblasts / cytology
Neovascularization, Physiologic
Pericytes / cytology
Rats
Rats, Inbred Strains
Sclerosis
Stem Cell Transplantation*

Substances

Antigens, Differentiation
Culture Media
Lectins
Lipoproteins, LDL
acetyl-LDL