Newly isolated alkalophilic Advenella species bioaugmented in activated sludge for high p-cresol removal

Eleni Xenofontos; Ana-Maria Tanase; Ileana Stoica; Ioannis Vyrides

doi:10.1016/j.nbt.2015.11.003

Newly isolated alkalophilic Advenella species bioaugmented in activated sludge for high p-cresol removal

N Biotechnol. 2016 Mar 25;33(2):305-10. doi: 10.1016/j.nbt.2015.11.003. Epub 2015 Nov 17.

Authors

Eleni Xenofontos¹, Ana-Maria Tanase², Ileana Stoica³, Ioannis Vyrides⁴

Affiliations

¹ Cyprus University of Technology, Department of Environmental Science and Technology, 95 Eirinis Str., P.O. BOX 50329, 3603 Lemesos, Cyprus.
² University of Bucharest, Department of Genetics, Intrarea Postocalelor nr. 1-3, OP 060101 Bucharest, Romania; University of Agronomic Science and Veterinary Medicine, Faculty of Biotechnologies, Bulevardul Marasti nr. 59, Bucharest, cod: 011464, Romania.
³ University of Bucharest, Department of Genetics, Intrarea Postocalelor nr. 1-3, OP 060101 Bucharest, Romania.
⁴ Cyprus University of Technology, Department of Environmental Science and Technology, 95 Eirinis Str., P.O. BOX 50329, 3603 Lemesos, Cyprus. Electronic address: Ioannis.vyrides@cut.ac.cy.

PMID: 26596887
DOI: 10.1016/j.nbt.2015.11.003

Abstract

In this work, an alkalophilic bacterium (LVX-4) capable of using p-cresol as sole source of carbon and energy was screened and isolated from soil polluted by used oil. Phylogenetic (16S rRNA) and phenotypic characterization using Biolog GN microplates and API 20NE strips indicated that LVX-4 strain is a new Advenella species. It showed both the capability to degrade of p-cresol at high concentrations (750 mg/L) and to use p-cresol for growth in a pH from 7 to 10, although the optimum pH was 9. Moreover bioaugmentation of activated sludge with this strain lead to the complete removal of p-cresol in less than 100 h. This is the first study that shows the potential of Advenella sp. to be bioaugmented in activated sludge system for p-cresol biodegradation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alcaligenaceae / classification
Alcaligenaceae / isolation & purification
Alcaligenaceae / metabolism*
Biodegradation, Environmental
Cresols / metabolism*
Petroleum Pollution
Phenotype
Phylogeny
RNA, Ribosomal, 16S / genetics
Sewage / microbiology*
Soil Microbiology*
Soil Pollutants / metabolism*

Substances

Cresols
RNA, Ribosomal, 16S
Sewage
Soil Pollutants
4-cresol