Cranberries (Oxycoccus quadripetalus) inhibit pro-inflammatory cytokine and chemokine expression in 3T3-L1 adipocytes

Food Chem. 2016 Apr 1:196:1137-43. doi: 10.1016/j.foodchem.2015.10.069. Epub 2015 Oct 19.

Abstract

Oxidative stress and inflammation are involved in the development of obesity, type 2 diabetes and vascular complications. Systemic inflammation, as seen in obesity, is associated with high plasmatic levels of pro-inflammatory, pro-atherogenic and pro-thrombotic adipokines. Here we studied the effects of lyophilized cranberries (LCB) on the secretion and expression of PAI-1, IL-6, MCP-1 and leptin in mature 3T3-L1 adipocytes under baseline conditions and excessive inflammatory response elicitation by stimulation with H2O2. Our data demonstrated that LCB significantly reduced the expression and secretion of IL-6, MCP-1 and leptin, as well as suppressed the overexpression of PAI-1 induced by H2O2. Our findings suggested that LCB counteracted the stimulatory effect of H2O2 on secretion and expression of pro-inflammatory adipokines, implying a potential anti-inflammatory effect during the inflammatory process induced via oxidative stress in adipose tissue.

Keywords: 3T3-L1 adipocytes; Chemokines; Cranberries; Cytokines; Inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / drug effects
  • Adipocytes / metabolism*
  • Animals
  • Anti-Inflammatory Agents / pharmacology
  • Cytokines / drug effects
  • Cytokines / metabolism*
  • Fruit*
  • Hydrogen Peroxide / pharmacology
  • Inflammation / chemically induced*
  • Inflammation / drug therapy
  • Leptin / metabolism
  • Mice
  • Plant Preparations / pharmacology*
  • Serpin E2 / drug effects
  • Serpin E2 / metabolism
  • Vaccinium*

Substances

  • Anti-Inflammatory Agents
  • Cytokines
  • Leptin
  • Plant Preparations
  • Serpin E2
  • Serpine2 protein, mouse
  • Hydrogen Peroxide