Optimization of a QuEChERS based method by means of central composite design for pesticide multiresidue determination in orange juice by UHPLC-MS/MS

Tiele M Rizzetti; Magali Kemmerich; Manoel L Martins; Osmar D Prestes; Martha B Adaime; Renato Zanella

doi:10.1016/j.foodchem.2015.09.010

Optimization of a QuEChERS based method by means of central composite design for pesticide multiresidue determination in orange juice by UHPLC-MS/MS

Food Chem. 2016 Apr 1:196:25-33. doi: 10.1016/j.foodchem.2015.09.010. Epub 2015 Sep 8.

Authors

Tiele M Rizzetti¹, Magali Kemmerich¹, Manoel L Martins¹, Osmar D Prestes¹, Martha B Adaime¹, Renato Zanella²

Affiliations

¹ Laboratório de Análise de Resíduos de Pesticidas (LARP), Departamento de Química, Universidade Federal de Santa Maria, CEP 97105-900 Santa Maria, RS, Brazil.
² Laboratório de Análise de Resíduos de Pesticidas (LARP), Departamento de Química, Universidade Federal de Santa Maria, CEP 97105-900 Santa Maria, RS, Brazil. Electronic address: rzanella@base.ufsm.br.

PMID: 26593461
DOI: 10.1016/j.foodchem.2015.09.010

Abstract

In this study, different extraction procedures based on the QuEChERS method were compared for the multiresidue determination of pesticides in orange juice by ultra high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). After choosing preliminary conditions, an experimental design was carried out with the variables C18, PSA, NaOH and CH3COONa to optimize the sample preparation step. The validation results of the validation were satisfactory, since the method presented recoveries between 70% and 118%, with RSD lower than 19% for spike levels between 10 and 100 μg L(-1). The method limit of detection (LOD) and limit of quantification (LOQ) ranged from 3.0 to 7.6 μg L(-1) and from 4.9 to 26 μg L(-1), respectively. The method developed was adequate for the determination of 74 pesticide residues in orange juice.

Keywords: Central composite design; Orange juice; Pesticides; QuEChERS; UHPLC–MS/MS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Beverages / analysis*
Chromatography, High Pressure Liquid / methods*
Citrus sinensis / chemistry*
Pesticide Residues / analysis
Pesticide Residues / chemistry*
Tandem Mass Spectrometry / methods*

Substances

Pesticide Residues