Multiple biomarkers biosensor with just-in-time functionalization: Application to prostate cancer detection

Biosens Bioelectron. 2016 Mar 15:77:1192-200. doi: 10.1016/j.bios.2015.10.064. Epub 2015 Oct 28.

Abstract

We present a novel lab-on-a-chip (LOC) device for the simultaneous detection of multiple biomarkers using simple voltage measurements. The biosensor functionalization is performed in-situ, immediately before its use, facilitating reagents storage and massive devices fabrication. Sensitivity, limit of detection (LOD) and limit of quantification (LOQ) are tunable depending on the in-chip flown sample volumes. As a proof-of-concept, the system has been tested and adjusted to quantify two proteins found in blood that are susceptible to be used combined, as a screening tool, to diagnose prostate cancer (PCa): prostate-specific antigen (PSA) and spondin-2 (SPON2). This combination of biomarkers has been reported to be more specific for PCa diagnostics than the currently accepted but rather controversial PSA indicator. The range of detection for PSA and SPON2 could be adjusted to the clinically relevant range of 1 to 10 ng/ml. The system was tested for specificity to the evaluated biomarkers. This multiplex system can be modified and adapted to detect a larger quantity of biomarkers, or different ones, of relevance to other specific diseases.

Keywords: Adjustable sensing; Impedance measurements; In situ functionalization; Microfluidics; Prostate specific antigen; Self-assembled monolayers.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / blood
  • Biosensing Techniques / instrumentation*
  • Complex Mixtures / blood
  • Computer Systems
  • Conductometry / instrumentation
  • Equipment Design
  • Equipment Failure Analysis
  • Extracellular Matrix Proteins / blood*
  • Humans
  • Immunoassay / instrumentation
  • Lab-On-A-Chip Devices*
  • Male
  • Neoplasm Proteins / blood*
  • Prostate-Specific Antigen / blood*
  • Prostatic Neoplasms / blood*
  • Prostatic Neoplasms / diagnosis*

Substances

  • Biomarkers, Tumor
  • Complex Mixtures
  • Extracellular Matrix Proteins
  • Neoplasm Proteins
  • SPON2 protein, human
  • Prostate-Specific Antigen