The steroid receptor RNA activator protein (SRAP) controls cancer cell migration/motility

Yi Yan; Charlton Cooper; Mohammad K Hamedani; Brent Guppy; Wayne Xu; Deborah Tsuyuki; Christine Zhang; Zoann Nugent; Anne Blanchard; James R Davie; Kirk McManus; Leigh C Murphy; Yvonne Myal; Etienne Leygue

doi:10.1016/j.febslet.2015.11.007

The steroid receptor RNA activator protein (SRAP) controls cancer cell migration/motility

FEBS Lett. 2015 Dec 21;589(24 Pt B):4010-8. doi: 10.1016/j.febslet.2015.11.007. Epub 2015 Nov 17.

Authors

Affiliations

¹ Manitoba Institute of Cell Biology, 675 McDermot Ave., R3E0V9 Winnipeg, Manitoba, Canada; Department of Biochemistry and Medical Genetics, University of Manitoba, 770 Bannatyne Avenue, R3E0W3 Winnipeg, Manitoba, Canada.
² Manitoba Institute of Cell Biology, 675 McDermot Ave., R3E0V9 Winnipeg, Manitoba, Canada.
³ Department of Immunology, University of Manitoba, 413 Apotex Center, 750 McDermot Ave., R3E0T5 Winnipeg, Manitoba, Canada.
⁴ Manitoba Institute of Cell Biology, 675 McDermot Ave., R3E0V9 Winnipeg, Manitoba, Canada; Department of Physiology, University of Manitoba, 770 Bannatyne Avenue, R3E0W3 Winnipeg, Manitoba, Canada.
⁵ Manitoba Institute of Cell Biology, 675 McDermot Ave., R3E0V9 Winnipeg, Manitoba, Canada; Department of Biochemistry and Medical Genetics, University of Manitoba, 770 Bannatyne Avenue, R3E0W3 Winnipeg, Manitoba, Canada. Electronic address: etienne.leygue@umanitoba.ca.

PMID: 26581859
DOI: 10.1016/j.febslet.2015.11.007

Abstract

The steroid receptor RNA activator gene (SRA1) produces both a functional RNA (SRA) and a protein (SRAP), whose exact physiological roles remain unknown. To identify cellular processes regulated by SRAP we compared the transcriptome of Hela and MDA-MB-231 cancer cells upon depletion of the SRA/SRAP transcripts or overexpression of the SRAP protein. RNA-seq and Ontology analyses pinpointed cellular movement as potentially regulated by SRAP. Using live cell imaging, we found that SRA/SRAP depletion and SRAP overexpression lead respectively to a decrease and increase in cancer cell motility. Our results highlight for the first time a link existing between SRA1 gene expression and cell motility.

Keywords: Cancer; Cell motility; Functional RNA; Non-coding RNA; Steroid receptor RNA activator; Steroid receptor RNA protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Breast Neoplasms / metabolism*
Breast Neoplasms / pathology
Carrier Proteins / antagonists & inhibitors
Carrier Proteins / genetics
Carrier Proteins / metabolism*
Cell Line, Tumor
Cell Movement
Female
Gene Expression Profiling
Gene Expression Regulation, Neoplastic*
Humans
Luminescent Proteins / genetics
Luminescent Proteins / metabolism
Microscopy, Video
Neoplasm Invasiveness
Neoplasm Proteins / antagonists & inhibitors
Neoplasm Proteins / genetics
Neoplasm Proteins / metabolism*
RNA Interference
RNA, Neoplasm / antagonists & inhibitors
RNA, Neoplasm / metabolism*
RNA, Small Interfering
Recombinant Fusion Proteins / chemistry
Recombinant Fusion Proteins / metabolism
Single-Cell Analysis
Uterine Cervical Neoplasms / metabolism*
Uterine Cervical Neoplasms / pathology

Substances

Carrier Proteins
Luminescent Proteins
Neoplasm Proteins
RNA, Neoplasm
RNA, Small Interfering
Recombinant Fusion Proteins
steroid receptor RNA activator, human