Bacterial polysaccharide levan as stabilizing, non-toxic and functional coating material for microelement-nanoparticles

Olesja M Bondarenko; Angela Ivask; Anne Kahru; Heiki Vija; Tiina Titma; Meeri Visnapuu; Urmas Joost; Ksenia Pudova; Signe Adamberg; Triinu Visnapuu; Tiina Alamäe

doi:10.1016/j.carbpol.2015.09.093

Bacterial polysaccharide levan as stabilizing, non-toxic and functional coating material for microelement-nanoparticles

Carbohydr Polym. 2016 Jan 20:136:710-20. doi: 10.1016/j.carbpol.2015.09.093. Epub 2015 Sep 30.

Authors

Olesja M Bondarenko¹, Angela Ivask², Anne Kahru³, Heiki Vija⁴, Tiina Titma⁵, Meeri Visnapuu⁶, Urmas Joost⁷, Ksenia Pudova⁸, Signe Adamberg⁹, Triinu Visnapuu¹⁰, Tiina Alamäe¹¹

Affiliations

¹ Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618 Tallinn, Estonia. Electronic address: olesja.bondarenko@kbfi.ee.
² Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618 Tallinn, Estonia. Electronic address: angela.ivask@kbfi.ee.
³ Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618 Tallinn, Estonia. Electronic address: anne.kahru@kbfi.ee.
⁴ Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618 Tallinn, Estonia. Electronic address: heiki.vija@kbfi.ee.
⁵ Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618 Tallinn, Estonia. Electronic address: tiina.titma@kbfi.ee.
⁶ Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618 Tallinn, Estonia; Institute of Physics, University of Tartu, Ravila 14c, 50411 Tartu, Estonia. Electronic address: meeri.visnapuu@ut.ee.
⁷ Institute of Physics, University of Tartu, Ravila 14c, 50411 Tartu, Estonia. Electronic address: urmas.joost@ut.ee.
⁸ Tallinn University of Technology, Ehitajate tee 5, 19086 Tallinn, Estonia; Competence Center of Food and Fermentation Technologies, Akadeemia tee 15A, 12618 Tallinn, Estonia. Electronic address: ksenia.kuksina@gmail.com.
⁹ Competence Center of Food and Fermentation Technologies, Akadeemia tee 15A, 12618 Tallinn, Estonia. Electronic address: signe.adamberg@ttu.ee.
¹⁰ Institute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010 Tartu, Estonia. Electronic address: triinu.visnapuu@ut.ee.
¹¹ Institute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010 Tartu, Estonia. Electronic address: talamae@ebc.ee.

PMID: 26572404
DOI: 10.1016/j.carbpol.2015.09.093

Abstract

Levan, fructose-composed biopolymer of bacterial origin, has potential in biotechnology due to its prebiotic and immunostimulatory properties. In this study levan synthesized by levansucrase from Pseudomonas syringae was thoroughly characterized and used as multifunctional biocompatible coating material for microelement-nanoparticles (NPs) of selenium, iron and cobalt. Transmission electron microscopy (TEM), hydrodynamic size measurements (DLS) and X-ray photoelectron spectroscopy (XPS) showed the interaction of levan with NPs. Levan stabilized the dispersions of NPs, decreased their toxicity and had protective effect on human intestinal cells Caco-2. In addition, levan attached to cobalt NPs remained accessible as a substrate for the colon bacteria Bacteroides thetaiotaomicron. We suggest that the combination of levan and nutritionally important microelements in the form of NPs serves as a first step towards a novel "2 in 1" approach for food supplements to provide safe and efficient delivery of microelements for humans and support beneficial gut microbiota with nutritional oligosaccharides.

Keywords: Bacteroides thetaiotaomicron; Caco-2 in vitro; Co, Fe and Se nanomaterial; Cobalt tetraoxide (PubChem CID: 6432046); Food supplement; Levan N (PubChem CID: 440946); Magnetic oxide (PubChem CID: 14789); Safe-by-design; Selenium (PubChem CID: 6326970).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteroides / drug effects
Caco-2 Cells
Coated Materials, Biocompatible / adverse effects
Coated Materials, Biocompatible / chemistry*
Coated Materials, Biocompatible / pharmacology
Enterocytes / drug effects
Fructans / adverse effects
Fructans / chemistry*
Fructans / pharmacology
Humans
Nanoparticles / chemistry*
Trace Elements / chemistry*

Substances

Coated Materials, Biocompatible
Fructans
Trace Elements
levan