Multifunctional Silica Nanoparticles for Covalent Immobilization of Highly Sensitive Proteins

Adv Mater. 2015 Dec 22;27(48):7945-50. doi: 10.1002/adma.201503935. Epub 2015 Nov 17.

Abstract

A convenient reverse micellar one-pot reaction yields multifunctional silica nanoparticles, which can be tailored to effectively suppress non-specific adsorption and, at the same time, enable efficient specific covalent immobilization of proteins. Using two highly sensitive proteins, it is demonstrated that the new particles provide a suitable microenvironment to maintain the protein's activity.

Keywords: biomolecules; immobilization; proteins; silica nanoparticles; surface chemistry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Biosensing Techniques
  • Glucose / analysis
  • Immobilized Proteins / chemistry*
  • Nanoparticles / chemistry*
  • Silicon Dioxide / chemistry*
  • Sulfhydryl Compounds / chemistry

Substances

  • Immobilized Proteins
  • Sulfhydryl Compounds
  • Silicon Dioxide
  • Glucose