Cloning and expression of trehalose-6-phosphate synthase 1 from Rhizopus oryzae

J Basic Microbiol. 2016 May;56(5):459-68. doi: 10.1002/jobm.201500425. Epub 2015 Nov 16.

Abstract

Trehalose is a reducing disaccharide acting as a protectant against environmental stresses in many organisms. In fungi, Trehalose-6-phosphate synthase 1 (TPS1) plays a key role in the biosynthesis of trehalose. In this study, a full-length cDNA from Rhizopus oryzae encoding TPS1 (designated as RoTPS1) was isolated. The RoTPS1 cDNA is composed of 2505 nucleotides and encodes a protein of 834 amino acids with a molecular mass of 97.8 kDa. The amino acid sequence of RoTPS1 has a relatively high homology with the TPS1s in several other filamentous fungi. RoTPS1 was cloned into Saccharomyces cerevisiae and secretively expressed.

Keywords: Cloning; Rhizopus oryzae; Trehalose; Trehalose-6-phosphate synthase.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • DNA, Fungal / genetics
  • Genes, Fungal / genetics
  • Glucosyltransferases / biosynthesis
  • Glucosyltransferases / genetics*
  • Rhizopus / enzymology*
  • Rhizopus / genetics*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism*
  • Sequence Analysis, DNA
  • Trehalose / biosynthesis*

Substances

  • DNA, Complementary
  • DNA, Fungal
  • Trehalose
  • Glucosyltransferases
  • trehalose-6-phosphate synthase