The existence of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA in mammals

Chem Commun (Camb). 2016 Jan 14;52(4):737-40. doi: 10.1039/c5cc07354e.

Abstract

We developed a novel strategy by oxidation-derivatization combined mass spectrometry analysis for the determination of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA. We reported the presence of 5-formylcytosine in RNA of mammals and found that ascorbic acid and hydroquinone can increase the oxidation of 5-methylcytosine to 5-hydroxymethylcytosine in DNA and RNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5-Methylcytosine / chemistry*
  • 5-Methylcytosine / metabolism
  • Animals
  • Cytosine / analogs & derivatives*
  • Cytosine / chemistry
  • Cytosine / metabolism
  • DNA / chemistry*
  • DNA / metabolism
  • DNA Methylation
  • Mammals
  • Mass Spectrometry
  • Oxidation-Reduction
  • RNA / chemistry*
  • RNA / metabolism

Substances

  • 5-formylcytosine
  • 5-hydroxymethylcytosine
  • RNA
  • 5-Methylcytosine
  • Cytosine
  • DNA