Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) and Bayesian phylogenetic analysis to characterize Candida clinical isolates

Silvia Angeletti; Alessandra Lo Presti; Eleonora Cella; Giordano Dicuonzo; Francesca Crea; Bernardetta Palazzotti; Etleva Dedej; Massimo Ciccozzi; Lucia De Florio

doi:10.1016/j.mimet.2015.11.003

Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) and Bayesian phylogenetic analysis to characterize Candida clinical isolates

J Microbiol Methods. 2015 Dec:119:214-22. doi: 10.1016/j.mimet.2015.11.003. Epub 2015 Nov 10.

Authors

Silvia Angeletti¹, Alessandra Lo Presti², Eleonora Cella², Giordano Dicuonzo³, Francesca Crea³, Bernardetta Palazzotti⁴, Etleva Dedej³, Massimo Ciccozzi², Lucia De Florio³

Affiliations

¹ Clinical Pathology and Microbiology Laboratory, University Hospital Campus Bio-Medico of Rome, Italy. Electronic address: s.angeletti@unicampus.it.
² Department of Infectious, Parasitic, and Immune-Mediated Diseases, Epidemiology Unit, Reference Centre on Phylogeny, Molecular Epidemiology, and Microbial Evolution (FEMEM), National Institute of Health, Rome, Italy.
³ Clinical Pathology and Microbiology Laboratory, University Hospital Campus Bio-Medico of Rome, Italy.
⁴ Laboratory of Clinical Microbiology, "S. Spirito" Hospital of Rome, Italy.

PMID: 26551247
DOI: 10.1016/j.mimet.2015.11.003

Abstract

Clinical Candida isolates from two different hospitals in Rome were identified and clustered by MALDI-TOF MS system and their origin and evolution estimated by Bayesian phylogenetic analysis. The different species of Candida were correctly identified and clustered separately, confirming the ability of these techniques to discriminate between different Candida species. Focusing MALDI-TOF analysis on a single Candida species, Candida albicans and Candida parapsilosis strains clustered differently for hospital setting as well as for period of isolation than Candida glabrata and Candida tropicalis isolates. The evolutionary rates of C. albicans and C. parapsilosis (1.93×10(-2) and 1.17×10(-2)substitutions/site/year, respectively) were in agreement with a higher rate of mutation of these species, even in a narrow period, than what was observed in C. glabrata and C. tropicalis strains (6.99×10(-4) and 7.52×10(-3)substitutions/site/year, respectively). C. albicans resulted as the species with the highest between and within clades genetic distance values in agreement with the temporal-related clustering found by MALDI-TOF and the high evolutionary rate 1.93×10(-2)substitutions/site/year.

Keywords: Candida isolates; Evolution; MALDI-TOF; Phylogenesis.

Publication types

Evaluation Study

MeSH terms

Bayes Theorem
Candida / chemistry
Candida / classification*
Candida / isolation & purification*
Candidiasis / microbiology*
Classification / methods*
Humans
Phylogeny*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
Tandem Mass Spectrometry / methods*