[Validation of an in-house method for the determination of zinc in serum: Meeting the requirements of ISO 17025]

Rev Calid Asist. 2015 Nov-Dec;30(6):319-26. doi: 10.1016/j.cali.2015.08.001. Epub 2015 Nov 3.
[Article in Spanish]

Abstract

Objective: The aim of this report is to propose a scheme for validation of an analytical technique according to ISO 17025.

Material and methods: According to ISO 17025, the fundamental parameters tested were: selectivity, calibration model, precision, accuracy, uncertainty of measurement, and analytical interference.

Results: A protocol has been developed that has been applied successfully to quantify zinc in serum by atomic absorption spectrometry.

Conclusion: It is demonstrated that our method is selective, linear, accurate, and precise, making it suitable for use in routine diagnostics.

Keywords: Accreditation; Acreditación; Cinc sérico; ISO 17025; Serum zinc; Validación; Validation.

Publication types

  • Validation Study

MeSH terms

  • Accreditation / standards
  • Blood Chemical Analysis / standards*
  • Calibration
  • Humans
  • International Agencies / standards
  • Observer Variation
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrophotometry, Atomic / instrumentation
  • Spectrophotometry, Atomic / methods*
  • Spectrophotometry, Atomic / standards
  • Zinc / blood*

Substances

  • Zinc