ATP-Mediated Compositional Change in Peripheral Myelin Membranes: A Comparative Raman Spectroscopy and Time-Of-Flight Secondary Ion Mass Spectrometry Study

Nikolay Kutuzov; Alexander Gulin; Vladimir Lyaskovskiy; Victor Nadtochenko; Georgy Maksimov

doi:10.1371/journal.pone.0142084

ATP-Mediated Compositional Change in Peripheral Myelin Membranes: A Comparative Raman Spectroscopy and Time-Of-Flight Secondary Ion Mass Spectrometry Study

PLoS One. 2015 Nov 6;10(11):e0142084. doi: 10.1371/journal.pone.0142084. eCollection 2015.

Authors

Nikolay Kutuzov¹, Alexander Gulin^{2

3}, Vladimir Lyaskovskiy⁴, Victor Nadtochenko^{2

3

5

6}, Georgy Maksimov¹

Affiliations

¹ Biophysics Department, Biological Faculty, Moscow State University, Leninskie gory 1/12, Moscow, Russian Federation, 119991.
² N.N. Semenov Institute of Chemical Physics, RAS, Kosigin str. 4, Moscow, Russian Federation, 119991.
³ Chemistry Faculty, Moscow State University, Leninskie Gory 1-3, Moscow, Russian Federation, 119991.
⁴ All-Russian Research Institute for Optical and Physical Measurements, Ozernaya 46, Moscow, Russian Federation, 119361.
⁵ Moscow Institute of Physics and Technology State University, Institutskiy per. 9, Dolgoprudny, Moscow Region, Russian Federation, 141700.
⁶ Institute of Problems of Chemical Physics RAS, Academician Semenov avenue 1, Chernogolovka, Moscow region, Russian Federation, 142432.

Abstract

In the present paper we addressed a mechanism of the myelin reorganization initiated by extracellular ATP and adenosine in sciatic nerves of the frog Rana temporaria. In combination with Raman microspectroscopy, allowing noninvasive live-cell measurements, we employed time-of-flight secondary ion mass spectrometry (TOF-SIMS) to follow the underlying changes in chemical composition of myelin membranes triggered by the purinergic agents. The simultaneous increase in lipid ordering degree, decrease in membrane fluidity and the degree of fatty acid unsaturation were induced by both ATP and adenosine. Mass spectrometry measurements revealed that ATP administration also led to the marked elevation of membrane cholesterol and decrease of phosphotidylcholine amounts. Vesicular lipid transport pathways are considered as possible mechanisms of compositional and structural changes of myelin.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphate / pharmacology*
Algorithms
Animals
Cell Membrane / metabolism*
Lipid Bilayers / metabolism*
Membrane Fluidity / drug effects
Models, Biological
Myelin Sheath / drug effects
Myelin Sheath / metabolism*
Rana temporaria
Sciatic Nerve / cytology
Sciatic Nerve / drug effects
Sciatic Nerve / metabolism*
Spectrometry, Mass, Secondary Ion / methods*
Spectrum Analysis, Raman / methods*

Substances

Lipid Bilayers
Adenosine Triphosphate

Grants and funding

The authors acknowledge financial support from Russian Foundation for Basic Research for young researchers, grant 14-04-31883-mol-a, and Presidium of the Russian Academy of Sciences, program “Basic sciences for medicine”. The TOF-SIMS measurements were carried out with partial support from the Russian Foundation for basic research grant 14-14-00856.