Microfluidic Image Cytometry for Single-Cell Phenotyping of Human Pluripotent Stem Cells

Yasumasa Mashimo; Ken-Ichiro Kamei

doi:10.1007/978-1-4939-2987-0_7

Microfluidic Image Cytometry for Single-Cell Phenotyping of Human Pluripotent Stem Cells

Methods Mol Biol. 2015:1346:85-98. doi: 10.1007/978-1-4939-2987-0_7.

Authors

Yasumasa Mashimo¹, Ken-Ichiro Kamei²

Affiliations

¹ Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 606-8501, Japan.
² Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, 606-8501, Japan. kkamei@icems.kyoto-u.ac.jp.

PMID: 26542717
DOI: 10.1007/978-1-4939-2987-0_7

Abstract

A microfluidic human pluripotent stem cell (hPSC) array has been developed for robust and reproducible hPSC culture methods to assess chemically defined serum- and feeder-free culture conditions. This microfluidic platform, combined with image cytometry, enables the systematic analysis of multiple simultaneously detected marker expression in individual cells, for screening of various chemically defined media across hPSC lines, and the study of phenotypic responses.

Keywords: Basic fibroblast growth factor; Chemically defined media; Human pluripotent stem cells; Immunofluorescence; Matrigel; Microfluidic hPSC array; Microfluidic image cytometry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques / instrumentation
Cell Line
Equipment Design
Fluorescent Antibody Technique / instrumentation*
Humans
Image Cytometry / instrumentation*
Micromanipulation / instrumentation
Phenotype
Pluripotent Stem Cells / cytology*
Single-Cell Analysis / instrumentation*