TRPC1 is required for survival and proliferation of cochlear spiral ganglion stem/progenitor cells

Int J Pediatr Otorhinolaryngol. 2015 Dec;79(12):2290-4. doi: 10.1016/j.ijporl.2015.10.027. Epub 2015 Oct 26.

Abstract

Objective: The present studies were designed to test the hypothesis that canonical transient receptor potential channel 1 (TRPC1) is required for the proliferation of cochlear spiral ganglion stem/progenitor cells (SPCs).

Methods and materials: TRPC1 were detected and evaluated in postnatal day 1 CBA/CaJ mice pups derived-cochlear spiral ganglion SPCs by reverse transcription-polymerase chain reaction, Western blot, immunocytochemistry, and calcium imaging. The cell viability and proliferation of the spiral ganglion SPCs following si-RNA mediated knockdown of TRPC1 or addition of TRPC channel blocker SKF9635 were compared to controls.

Results: In spiral ganglion SPCs, TRPC1 was found to be the most abundantly expressed TRPC subunit and shown to contribute to store-operated calcium entry. Silencing of TRPC1 or addition of TRPC channel blockers significantly decreased the rate of cell proliferation.

Conclusion: The results suggest that TRPC1 might serve as an essential molecule in regulating the proliferation of spiral ganglion SPCs.

Keywords: Canonical transient receptor potential channel; Cochlea; Proliferation; Spiral ganglion neuron; Stem/progenitor cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Cell Proliferation / physiology
  • Cell Survival / physiology
  • Cochlea / cytology*
  • Gene Silencing
  • Mice, Inbred CBA
  • Reverse Transcriptase Polymerase Chain Reaction
  • Spiral Ganglion / cytology*
  • Stem Cells / physiology*
  • TRPC Cation Channels / genetics
  • TRPC Cation Channels / physiology*

Substances

  • TRPC Cation Channels
  • transient receptor potential cation channel, subfamily C, member 1