Production, extraction and stabilization of lutein from microalga Chlorella sorokiniana MB-1

Chun-Yen Chen; Jesisca; Chienyan Hsieh; Duu-Jong Lee; Chien-Hsiang Chang; Jo-Shu Chang

doi:10.1016/j.biortech.2015.10.071

Production, extraction and stabilization of lutein from microalga Chlorella sorokiniana MB-1

Bioresour Technol. 2016 Jan:200:500-5. doi: 10.1016/j.biortech.2015.10.071. Epub 2015 Oct 24.

Authors

Chun-Yen Chen¹, Jesisca², Chienyan Hsieh³, Duu-Jong Lee⁴, Chien-Hsiang Chang², Jo-Shu Chang⁵

Affiliations

¹ University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
² Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
³ Department of Biotechnology, National Kaohsiung Normal University, Kaohsiung, Taiwan.
⁴ Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
⁵ Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan; Research Center for Energy Technology and Strategy Center, National Cheng Kung University, Tainan, Taiwan. Electronic address: changjs@mail.ncku.edu.tw.

PMID: 26519703
DOI: 10.1016/j.biortech.2015.10.071

Abstract

The efficiencies of extraction and preservation of lutein from microalgae are critical for the success of its commercialization. In this study, lutein was produced by Chlorella sorokiniana MB-1 via semi-batch mixotrophic cultivation. The microalgal biomass with a lutein content of 5.21mg/g was pretreated by bead-beating and high pressure cell disruption methods, and the lutein content was harvested by a reduced pressure extraction method. The effect of pretreatment, pressure, solvent type, extraction time and temperature on lutein recovery was investigated. Using high pressure pretreatment followed by extraction with tetrahydrofuran (THF) as solvent resulted in high lutein recovery efficiencies of 87.0% (20min) and 99.5% (40min) at 850mbar and 25°C. In contrast, using ethanol as the solvent, 86.2% lutein recovery was achieved under 450mbar, 35°C and 40min extraction. The extracted lutein was stabilized in olive oil or sunflower oil with half-lives of 53.1 and 63.8days, respectively.

Keywords: Chlorella sorokiniana; Lutein; Microalgae; Reduced pressure extraction; Stabilization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomass*
Chlorella / metabolism*
Furans / chemistry
Lutein / biosynthesis*
Lutein / chemistry
Microalgae / metabolism*
Olive Oil
Plant Oils
Pressure
Solvents / chemistry
Sunflower Oil
Temperature

Substances

Furans
Olive Oil
Plant Oils
Solvents
Sunflower Oil
tetrahydrofuran
Lutein