Laccase-syringaldehyde-mediated degradation of trace organic contaminants in an enzymatic membrane reactor: Removal efficiency and effluent toxicity

Luong N Nguyen; Jason P van de Merwe; Faisal I Hai; Frederic D L Leusch; Jinguo Kang; William E Price; Felicity Roddick; Saleh F Magram; Long D Nghiem

doi:10.1016/j.biortech.2015.10.054

Laccase-syringaldehyde-mediated degradation of trace organic contaminants in an enzymatic membrane reactor: Removal efficiency and effluent toxicity

Bioresour Technol. 2016 Jan:200:477-84. doi: 10.1016/j.biortech.2015.10.054. Epub 2015 Oct 19.

Authors

Luong N Nguyen¹, Jason P van de Merwe², Faisal I Hai³, Frederic D L Leusch², Jinguo Kang⁴, William E Price⁵, Felicity Roddick⁶, Saleh F Magram⁷, Long D Nghiem¹

Affiliations

¹ Strategic Water Infrastructure Lab, School of Civil, Mining and Environmental Engineering, University of Wollongong, Wollongong, NSW 2522, Australia.
² Smart Water Research Centre, Australian Rivers Institute, School of Environment, Griffith University, QLD 4222, Australia.
³ Strategic Water Infrastructure Lab, School of Civil, Mining and Environmental Engineering, University of Wollongong, Wollongong, NSW 2522, Australia. Electronic address: faisal@uow.edu.au.
⁴ Strategic Water Infrastructure Lab, School of Civil, Mining and Environmental Engineering, University of Wollongong, Wollongong, NSW 2522, Australia; Strategic Water Infrastructure Lab, School of Chemistry, University of Wollongong, Wollongong, NSW 2522, Australia.
⁵ Strategic Water Infrastructure Lab, School of Chemistry, University of Wollongong, Wollongong, NSW 2522, Australia.
⁶ School of Civil, Environmental and Chemical Engineering, RMIT University, Melbourne, VIC 3001, Australia.
⁷ Department of Civil Engineering, King Abdul Aziz University, Jeddah 21589, Saudi Arabia.

PMID: 26519700
DOI: 10.1016/j.biortech.2015.10.054

Abstract

Redox-mediators such as syringaldehyde (SA) can improve laccase-catalyzed degradation of trace organic contaminants (TrOCs) but may increase effluent toxicity. The degradation performance of 14 phenolic and 17 non-phenolic TrOCs by a continuous flow enzymatic membrane reactor (EMR) at different TrOC and SA loadings was assessed. A specific emphasis was placed on the investigation of the toxicity of the enzyme (laccase), SA, TrOCs and the treated effluent. Batch tests demonstrated significant individual and interactive toxicity of the laccase and SA preparations. Reduced removal of resistant TrOCs by the EMR was observed for dosages over 50μg/L. SA addition at a concentration of 10μM significantly improved TrOC removal, but no removal improvement was observed at the elevated SA concentrations of 50 and 100μM. The treated effluent showed significant toxicity at SA concentrations beyond 10μM, providing further evidence that higher dosage of SA must be avoided.

Keywords: Enzymatic membrane reactor (EMR); Laccase; Syringaldehyde; Toxicity; Trace organic contaminants (TrOCs).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aspergillus oryzae
Benzaldehydes / chemistry*
Biodegradation, Environmental
Bioreactors*
Catalysis
Clostridium
Dose-Response Relationship, Drug
Inhibitory Concentration 50
Kinetics
Laccase / metabolism*
Luminescence
Membranes, Artificial*
Organic Chemicals / chemistry*
Oxidation-Reduction
Photobacterium

Substances

Benzaldehydes
Membranes, Artificial
Organic Chemicals
syringaldehyde
Laccase