Structural characterization and anti-tumor activity of polysaccharide produced by Hirsutella sinensis

Int J Biol Macromol. 2016 Jan:82:959-66. doi: 10.1016/j.ijbiomac.2015.10.075. Epub 2015 Oct 27.

Abstract

HSP-III, a novel homogeneous polysaccharide with 513.89 kDa molecular weight, was fractionated from submerged cultures of Hirsutella sinensis by Sevag and chromatography on Sephadex G-100 column. The total sugar content of HSP-III was amounted to 89.87%. Based on the results of high performance gel permeation chromatogram (HPGPC), FT-IR, NMR spectroscopy, GC, periodate oxidation-smith degradation and methylation analysis, it showed that HSP-III was mainly composed of mannose and galactose, and a small amount of rhamnose, arabinose, xylose, and glucose. The molar ratio of Rha:Ara:Xyl:Man:Glu:Gal was 1.00:2.44:13.11:74.13:13.80:54.39. The main chain of HSP-III was majorly composed of (1→3) glucose. The tumor inhibition ratio on H22 cell was 79.04% at 100 μg/mL of HSP-III.

Keywords: Anti-tumor activity; Polysaccharide; Structure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / chemistry*
  • Antineoplastic Agents / isolation & purification
  • Antineoplastic Agents / pharmacology*
  • Ascomycota* / metabolism
  • Ascomycota* / ultrastructure
  • Cell Line, Tumor
  • Cell Survival
  • Fungal Polysaccharides / chemistry*
  • Fungal Polysaccharides / isolation & purification
  • Fungal Polysaccharides / pharmacology*
  • Humans
  • Magnetic Resonance Spectroscopy
  • Methylation
  • Molecular Weight
  • Periodic Acid / chemistry
  • Spectroscopy, Fourier Transform Infrared
  • Structure-Activity Relationship

Substances

  • Antineoplastic Agents
  • Fungal Polysaccharides
  • Periodic Acid
  • metaperiodate