Construction of Commercial Sweet Cherry Linkage Maps and QTL Analysis for Trunk Diameter

Jing Wang; Kaichun Zhang; Xiaoming Zhang; Guohua Yan; Yu Zhou; Laibao Feng; Yang Ni; Xuwei Duan

doi:10.1371/journal.pone.0141261

Construction of Commercial Sweet Cherry Linkage Maps and QTL Analysis for Trunk Diameter

PLoS One. 2015 Oct 30;10(10):e0141261. doi: 10.1371/journal.pone.0141261. eCollection 2015.

Authors

Jing Wang¹, Kaichun Zhang¹, Xiaoming Zhang¹, Guohua Yan¹, Yu Zhou¹, Laibao Feng², Yang Ni¹, Xuwei Duan¹

Affiliations

¹ Institute of Forestry and Pomology, Beijing Academy of Agricultural and Forestry Sciences, Beijing, 100093, China; Beijing Engineering Research Center for Deciduous Fruit Trees, Beijing, 100093, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, P.R. China, Beijing, 100093, China.
² Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Xiangshan Nanxincun 20, Beijing, 100093, China.

Abstract

A cross between the sweet cherry (Prunus avium) cultivars 'Wanhongzhu' and 'Lapins' was performed to create a mapping population suitable for the construction of a linkage map. The specific-locus amplified fragment (SLAF) sequencing technique used as a single nucleotide polymorphism (SNP) discovery platform and generated 701 informative genotypic assays; these, along with 16 microsatellites (SSRs) and the incompatibility (S) gene, were used to build a map which comprised 8 linkage groups (LGs) and covered a genetic distance of 849.0 cM. The mean inter-marker distance was 1.18 cM and there were few gaps > 5 cM in length. Marker collinearity was maintained with the established peach genomic sequence. The map was used to show that trunk diameter (TD) is under the control of 4 loci, mapping to 3 different LGs. Different locus influenced TD at a varying stage of the tree's development. The high density 'W×L' genetic linkage map has the potential to enable high-resolution identification of QTLs of agronomically relevant traits, and accelerate sweet cherry breeding.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Mapping / methods*
Genes, Plant
Genotype
Plant Breeding
Plant Stems / anatomy & histology*
Plant Stems / genetics
Polymorphism, Single Nucleotide
Prunus avium / anatomy & histology*
Prunus avium / genetics
Quantitative Trait Loci*
Sequence Analysis, DNA

Associated data

SRA/SRP063722

Grants and funding

This work was supported by the National Natural Science Foundation of China (31401827); URL: (http://www.nsfc.gov.cn/). JW received the funding. The funder had a role in study design, data collection and analysis, and decision to publish. This work was supported by the National Natural Science Foundation of China (31272123); URL: (http://www.nsfc.gov.cn/). KCZ received the funding. The funder had a role in the decision to publish. This work was supported by the Special Fund for the Construction of Scientific and Technological Innovation Capability (KJCX201101009, KJCX201004003, KJCX20140110); URL: (http://www.baafs.net.cn/). KCZ received these funding. The funder had a role in the decision to publish. This work was supported by the National Plan for Science, Technology and Innovation (2013BAD02B03-1-04); URL: (http://program.most.gov.cn/). KCZ received these funding. The funder had role in decision to publish. This work was supported by the Key Project of Beijing Municipal Science and Technology Commission (D131100000113001); URL: (http://www.bjkw.gov.cn). KCZ received these funding. The funder had a role in the decision to publish. This work was supported by the National Crop Germplasm Resources Platform Project (2012-068); URL: (http://www.most.gov.cn/). GHY received these funding. The funder had a role in data collection and analysis.