Use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) to detect carbapenemase production in Enterobacteriaceae by a rapid meropenem degradation assay

New Microbiol. 2015 Oct;38(4):571-6. Epub 2015 Oct 20.

Abstract

We evaluated the analytical performance of a liquid chromatography-tandem mass spectrometry assay to detect carbapenemase activity in a group of carbapenemase-producing Enterobacteriaceae by meropenem hydrolysis. This one-hour method showed a sensitivity of 94% and a specificity of 100%, representing a rapid and reliable option compared to conventional phenotypic assays.

Keywords: Carbapenem resistance; Carbapenemase activity; Enterobacteriaceae; Liquid chromatography-tandem mass spectrometry; Meropenem hydrolysis.

Publication types

  • Evaluation Study

MeSH terms

  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / metabolism*
  • Bacterial Proteins / analysis
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / metabolism*
  • Biocatalysis
  • Chromatography, Liquid / methods*
  • Enterobacteriaceae / chemistry
  • Enterobacteriaceae / enzymology*
  • Enterobacteriaceae / isolation & purification
  • Enterobacteriaceae / metabolism
  • Enterobacteriaceae Infections / microbiology*
  • Humans
  • Meropenem
  • Tandem Mass Spectrometry / methods*
  • Thienamycins / chemistry
  • Thienamycins / metabolism*
  • beta-Lactamases / chemistry*
  • beta-Lactamases / metabolism

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Thienamycins
  • beta-Lactamases
  • carbapenemase
  • Meropenem