Ectopic Bone Formation by Mesenchymal Stem Cells Derived from Human Term Placenta and the Decidua

Gina D Kusuma; Danijela Menicanin; Stan Gronthos; Ursula Manuelpillai; Mohamed H Abumaree; Mark D Pertile; Shaun P Brennecke; Bill Kalionis

doi:10.1371/journal.pone.0141246

Ectopic Bone Formation by Mesenchymal Stem Cells Derived from Human Term Placenta and the Decidua

PLoS One. 2015 Oct 20;10(10):e0141246. doi: 10.1371/journal.pone.0141246. eCollection 2015.

Authors

Gina D Kusuma¹, Danijela Menicanin², Stan Gronthos³, Ursula Manuelpillai⁴, Mohamed H Abumaree⁵, Mark D Pertile⁶, Shaun P Brennecke¹, Bill Kalionis¹

Affiliations

¹ Department of Obstetrics and Gynaecology, Royal Women's Hospital, The University of Melbourne, Parkville, Victoria, Australia; Pregnancy Research Centre, Department of Perinatal Medicine, Royal Women's Hospital, Parkville, Victoria, Australia.
² Mesenchymal Stem Cell Laboratory, Faculty of Health Sciences, School of Medical Sciences, University of Adelaide, Adelaide, Australia; Colgate Australian Clinical Dental Research Centre, School of Dentistry, University of Adelaide, Adelaide, Australia.
³ Mesenchymal Stem Cell Laboratory, Faculty of Health Sciences, School of Medical Sciences, University of Adelaide, Adelaide, Australia.
⁴ Pregnancy Research Centre, Department of Perinatal Medicine, Royal Women's Hospital, Parkville, Victoria, Australia; Centre for Genetic Diseases, Monash Institute of Medical Research-Prince Henry's Institute of Medical Research and Monash University, Clayton, Victoria, Australia.
⁵ King Abdullah International Medical Research Center/ King Saud Bin Abdulaziz University for Health Sciences, College of Science and Health Professions, King Abdulaziz Medical City-National Guard Health Affairs, Riyadh, Kingdom of Saudi Arabia.
⁶ Victorian Clinical Genetics Services (VCGS), Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia; Department of Paediatrics, Royal Children's Hospital, The University of Melbourne, Parkville, Victoria, Australia.

Abstract

Mesenchymal stem cells (MSCs) are one of the most attractive cell types for cell-based bone tissue repair applications. Fetal-derived MSCs and maternal-derived MSCs have been isolated from chorionic villi of human term placenta and the decidua basalis attached to the placenta following delivery, respectively. Chorionic-derived MSCs (CMSCs) and decidua-derived MSCs (DMSCs) generated in this study met the MSCs criteria set by International Society of Cellular Therapy. These criteria include: (i) adherence to plastic; (ii) >90% expression of CD73, CD105, CD90, CD146, CD44 and CD166 combined with <5% expression of CD45, CD19 and HLA-DR; and (iii) ability to differentiate into osteogenic, adipogenic, and chondrogenic lineages. In vivo subcutaneous implantation into SCID mice showed that both bromo-deoxyuridine (BrdU)-labelled CMSCs and DMSCs when implanted together with hydroxyapatite/tricalcium phosphate particles were capable of forming ectopic bone at 8-weeks post-transplantation. Histological assessment showed expression of bone markers, osteopontin (OPN), osteocalcin (OCN), biglycan (BGN), bone sialoprotein (BSP), and also a marker of vasculature, alpha-smooth muscle actin (α-SMA). This study provides evidence to support CMSCs and DMSCs as cellular candidates with potent bone forming capacity.

MeSH terms

Animals
Biomarkers / metabolism
Cell Differentiation*
Cells, Cultured
Decidua / cytology*
Decidua / physiology
Female
Flow Cytometry
Humans
In Situ Hybridization, Fluorescence
Mesenchymal Stem Cell Transplantation*
Mesenchymal Stem Cells / cytology*
Mesenchymal Stem Cells / physiology
Mice
Mice, SCID
Osteogenesis / physiology*
Placenta / cytology*
Placenta / physiology
Pregnancy

Substances

Biomarkers

Grants and funding

BK and SPB were supported by grants from King Abdullah International Medical Research Centre (Grant No. RC08/114) and the Royal Women’s Hospital Foundation. GK was supported by Australian Postgraduate Award and an Australian Stem Cell Centre Postgraduate Scholarship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.