Supporting data of spatiotemporal proliferation of human stromal cells adjusts to nutrient availability and leads to stanniocalcin-1 expression in vitro and in vivo

Gustavo A Higuera; Hugo Fernandes; Tim W G M Spitters; Jeroen van de Peppel; Nils Aufferman; Roman Truckenmueller; Maryana Escalante; Reinout Stoop; Johannes P van Leeuwen; Jan de Boer; Vinod Subramaniam; Marcel Karperien; Clemens van Blitterswijk; Anton van Boxtel; Lorenzo Moroni

doi:10.1016/j.dib.2015.08.012

Supporting data of spatiotemporal proliferation of human stromal cells adjusts to nutrient availability and leads to stanniocalcin-1 expression in vitro and in vivo

Data Brief. 2015 Aug 28:5:84-94. doi: 10.1016/j.dib.2015.08.012. eCollection 2015 Dec.

Authors

Affiliations

¹ Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands.
² Erasmus Medical Center, Internal Medicine, Wytemaweg 80, 3015 CN Rotterdam, The Netherlands.
³ Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands ; Department of Complex Tissue Regeneration, MERLN Institute for Technology Inspired Regenerative Medicine, Universiteitsingel 40, 6229ER Maastricht, The Netherlands.
⁴ Biophysical Engineering Group, Mesa Institute for Nanotechnology, University of Twente, Drienerlolaan 5, 7522 NB Enschede, The Netherlands.
⁵ TNO, Metabolic Health Research, Zernikedreef 9, 2333 CK Leiden, The Netherlands.
⁶ Department of Tissue Regeneration, MIRA - Institute for Biomedical Technology, University of Twente, Drienerlolaan 5, 7522 NB, Enschede, The Netherlands ; Department of cell biology inspired tissue engineering, MERLN Institute for Technology Inspired Regenerative Medicine, Universiteitsingel 40, 6229ER Maastricht, The Netherlands.
⁷ Systems and Control Group, Wageningen University, PO Box 17, 6700 AA Wageningen, The Netherlands.

Abstract

This data article contains seven figures and two tables supporting the research article entitled: spatiotemporal proliferation of human stromal cells adjusts to nutrient availability and leads to stanniocalcin-1 expression in vitro and in vivo[1]. The data explain the culture of stromal cells in vitro in three culture systems: discs, scaffolds and scaffolds in a perfusion bioreactor system. Also, quantification of extracellular matrix components (ECM) in vitro and staining of ECM components in vivo can be found here. Finally the quantification of blood vessels dimensions from CD31 signals and representative histograms of stanniocalcin-1 fluorescent signals in negative controls and experimental conditions in vivo are presented.