Gene expression profiling of WDR5 regulated genes in bladder cancer

Xu Chen; Peng Gu; Kuiqing Li; Weibin Xie; Changhao Chen; Tianxin Lin; Jian Huang

doi:10.1016/j.gdata.2015.05.003

Gene expression profiling of WDR5 regulated genes in bladder cancer

Genom Data. 2015 May 14:5:27-9. doi: 10.1016/j.gdata.2015.05.003. eCollection 2015 Sep.

Authors

Xu Chen¹, Peng Gu¹, Kuiqing Li², Weibin Xie², Changhao Chen², Tianxin Lin¹, Jian Huang²

Affiliations

¹ Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China ; Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
² Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.

Abstract

WD repeat domain 5 (WDR5) plays an important role in various biological functions through the epigenetic regulation of gene transcription (Wysocka et al., 2005 [1]; Sandstrom et al., 2014[2]; Ang et al., 2011[3]). Recently, our study found that WDR5 was upregulated in bladder cancer tissues, promoted bladder cancer cell proliferation, self-renewal and chemoresistance to cisplatin in bladder cancer cells in vitro, and tumor growth in vivo (Chen et al., 2015). To gain a molecular understanding of the role of WDR5 in promoting bladder cancer, we performed a genome-wide analysis on WDR5 knockdown by microarray gene expression profiling. Here we provide detailed experimental methods and analysis for the microarray data, which have been deposited into Gene Expression Omnibus (GEO): GSE59132.

Keywords: Bladder cancer; Microarrays; Transcriptional profiling; WDR5.