Illumina-based analysis of bacterial community in Khuangcherapuk cave of Mizoram, Northeast India

Surajit De Mandal; Amrita Kumari Panda; Esther Lalnunmawii; Satpal Singh Bisht; Nachimuthu Senthil Kumar

doi:10.1016/j.gdata.2015.04.023

Illumina-based analysis of bacterial community in Khuangcherapuk cave of Mizoram, Northeast India

Genom Data. 2015 May 8:5:13-4. doi: 10.1016/j.gdata.2015.04.023. eCollection 2015 Sep.

Authors

Surajit De Mandal¹, Amrita Kumari Panda¹, Esther Lalnunmawii¹, Satpal Singh Bisht¹, Nachimuthu Senthil Kumar¹

Affiliation

¹ Department of Biotechnology, Mizoram University, Aizawl 796004, Mizoram, India.

Abstract

Bacterial community of the Khuangcherapuk cave sediment was assessed by Illumina amplicon sequencing. The metagenome comprised of 533,120 raw reads with an average base quality (Phred score) 36.75 and G + C content is 57.61%. A total of 18 bacterial phyla were detected with following abundant genus - Mycobacterium (21.72%), Rhodococcus (7.09%), Alteromonas (1.42%), Holomonas (0.7%) and Salinisphaera (0.20%). Majority portion of the sequences (68%) is unclassified at the genus level indicating the possibilities for the presence of novel species in this cave. This study reports the cave bacterial diversity from the biodiversity hotspot region of Eastern Himalayas. Metagenome sequence data are available at NCBI under the Bioproject database with accession no. SRP056890.

Keywords: Illumina; Khuangcherapuk cave; Metagenome.