Transcriptome analysis of Rpl11-deficient zebrafish model of Diamond-Blackfan Anemia

Zhaojun Zhang; Haibo Jia; Qian Zhang; Yang Wan; Binfeng Song; Qiong Jia; Hanzhi Liu; Xiaofan Zhu; Xiangdong Fang

doi:10.1016/j.gdata.2014.06.008

Transcriptome analysis of Rpl11-deficient zebrafish model of Diamond-Blackfan Anemia

Genom Data. 2014 Jun 17:2:173-5. doi: 10.1016/j.gdata.2014.06.008. eCollection 2014 Dec.

Authors

Zhaojun Zhang¹, Haibo Jia², Qian Zhang¹, Yang Wan³, Binfeng Song², Qiong Jia², Hanzhi Liu³, Xiaofan Zhu³, Xiangdong Fang¹

Affiliations

¹ CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
² Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
³ State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.

Abstract

To comprehensively reflect the roles of Rpl11 on the transcriptome of zebrafish model of Diamond-Blackfan Anemia (DBA), we performed whole-genome transcriptome sequencing on the Illumina Hi-Seq 2000 sequencing platform. Two different transcriptomes of zebrafish Rpl11-deficient and control Morpholino (Mo) embryos were collected and analyzed. The experimental design and methods, including sample preparation, RNA-Seq data evaluation and treatment, were described in details so that representative high-throughput sequencing data were acquired for assessing the actual impacts of Rpl11 on zebrafish embryos. We provided the accession number GSE51326 for easy access to the database.

Keywords: DBA; High-Seq 2000; IPA; Transcriptome; Zebrafish.