Regulatory Elements in Vectors for Efficient Generation of Cell Lines Producing Target Proteins

O Maksimenko; N B Gasanov; P Georgiev

Regulatory Elements in Vectors for Efficient Generation of Cell Lines Producing Target Proteins

Acta Naturae. 2015 Jul-Sep;7(3):15-26.

Authors

O Maksimenko¹, N B Gasanov¹, P Georgiev¹

Affiliation

¹ Institute of Gene Biology, Russian Academy of Sciences, Vavilova str. 34/5, 119334, Moscow, Russia.

PMID: 26483956
PMCID: PMC4610161

Abstract

To date, there has been an increasing number of drugs produced in mammalian cell cultures. In order to enhance the expression level and stability of target recombinant proteins in cell cultures, various regulatory elements with poorly studied mechanisms of action are used. In this review, we summarize and discuss the potential mechanisms of action of such regulatory elements.

Keywords: S/MAR; STAR; UCOE; insulators; protein production in mammalian cells; recombinant proteins.