This study looks at the performance of a periodic microstructure surface on multiple colored light-emitting diodes (LEDs). A periodic microstructure surface on the package was used to reshape the light from the different colored LED chips in the multiple colored LEDs, which decreased the color separation and color nonuniformity of the multiple colored LEDs. Experimental results show that the multiple colored LEDs with a periodic microstructure surface could achieve medium or high collimation as well as good color uniformity. Thus, this study provides a simple and cost-effective method to greatly improve the color uniformity of multiple colored LEDs with medium or high collimation.