Effect of β-cyclodextrin on the internalization of nanoparticles into intestine epithelial cells

Eur J Pharm Sci. 2016 Jan 1:81:113-8. doi: 10.1016/j.ejps.2015.10.012. Epub 2015 Oct 23.

Abstract

The influence of β-cyclodextrin on the interaction and internalization of PLGA nanoparticles into intestine epithelial cells was assessed. For this purpose β-cyclodextrin was adsorbed on PLGA nanoparticles. Interaction of nanoparticles with Caco-2 cells, determined by fluorescence, was expressed as the number of particles per cell. Confocal microscopy confirmed the localization of the particles in the cell monolayer. The results showed that adsorption of β-cyclodextrin on the surface of PLGA nanoparticles reduces interaction with mucin, enhancing in this way the internalization into the Caco-2 cells.

Keywords: Caco-2 cells; Nanoparticles; PLGA; β-Cyclodextrin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Transport
  • Caco-2 Cells
  • Cell Nucleus / metabolism
  • Cell Survival / drug effects
  • Cytoplasm / metabolism
  • Epithelial Cells / metabolism*
  • Humans
  • Intestines / cytology
  • Lactic Acid / administration & dosage*
  • Lactic Acid / chemistry
  • Mucins / metabolism
  • Nanoparticles / administration & dosage*
  • Nanoparticles / chemistry
  • Polyglycolic Acid / administration & dosage*
  • Polyglycolic Acid / chemistry
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • beta-Cyclodextrins / administration & dosage*
  • beta-Cyclodextrins / chemistry

Substances

  • Mucins
  • beta-Cyclodextrins
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Lactic Acid
  • betadex