Potentiometric chemosensor for neopterin, a cancer biomarker, using an electrochemically synthesized molecularly imprinted polymer as the recognition unit

Biosens Bioelectron. 2016 Mar 15:77:565-72. doi: 10.1016/j.bios.2015.10.013. Epub 2015 Nov 14.

Abstract

With an established procedure of molecular imprinting, a synthetic polymer receptor for the neopterin cancer biomarker was devised and used as a recognition unit of a potentiometric chemosensor. For that, bis-bithiophene derivatized with cytosine and bithiophene derivatized with boronic acid were used as functional monomers. The open-circuit potential (OCP) based transduction under flow-injection analysis conditions (FIA) determined neopterin in the concentration range of 0.15-2.5mM with the 22 µM limit of detection (LOD) and 7.01(±0.15) mVmM(-1) sensitivity indicating its potential suitability in clinical analysis applications. The molecularly imprinted polymer (MIP) film showed an appreciable apparent imprinting factor of ~6. The chemosensor successfully discriminated the interferences including the 6-biopterin and pterin structural analogs of neopterin as well as glucose and creatinine. Moreover, it determined neopterin in synthetic serum samples.

Keywords: Cancer biomarker; Molecularly imprinted polymer; Neopterin; Open-circuit potential; Potentiometric chemosensor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biomarkers, Tumor / analysis*
  • Conductometry / instrumentation*
  • Electroplating / methods
  • Equipment Design
  • Equipment Failure Analysis
  • Molecular Imprinting / methods*
  • Neopterin / blood*
  • Polymers / chemistry*
  • Potentiometry / instrumentation*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Surface Properties

Substances

  • Biomarkers, Tumor
  • Polymers
  • Neopterin