Analysis of trace levels of sulfonamides in fish tissue using micro-scale pipette tip-matrix solid-phase dispersion and fast liquid chromatography tandem mass spectrometry

Food Chem. 2016 Mar 1:194:508-15. doi: 10.1016/j.foodchem.2015.08.050. Epub 2015 Aug 18.

Abstract

A micro-scale matrix solid-phase dispersion (MSPD) technique, using hydrophilic-lipophilic balance (HLB) material as sorbent and a pipette tip (PT) as the cartridge, was developed for the extraction and purification of sulfonamides in fish tissue. Eluates from PT-MSPD were analyzed using fast liquid chromatography and tandem mass spectrometry (LC-MS/MS). The method was fully validated; good linearity was obtained with correlation coefficients greater than 0.99. Precision and accuracy (RSD%) were in the range 1.4-10.3% while mean recoveries were 70.6-95.5%. With this technique, 15 aquatic samples (Collichthys niveatus) were analyzed for sulfonamides. The whole procedure took only 13min (5min for PT-MSPD and 8min for LC), materials for each sample included 5.1mL solvents (0.3mL for PT-MSPD and 4.8mL for LC), and 20mg HLB sorbent. Generally speaking, this method is indeed practical and particularly suitable for widespread drug residue analysis.

Keywords: Liquid chromatography/mass spectrometry; Pipette-tip matrix solid-phase dispersion; Sulfonamides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography, Liquid / methods*
  • Fishes / blood*
  • Solid Phase Extraction / methods*
  • Sulfonamides / analysis
  • Sulfonamides / metabolism*
  • Tandem Mass Spectrometry / methods*

Substances

  • Sulfonamides