Crystal Structure of the Conserved Amino Terminus of the Extracellular Domain of Matrix Protein 2 of Influenza A Virus Gripped by an Antibody

Ki Joon Cho; Bert Schepens; Kristof Moonens; Lei Deng; Walter Fiers; Han Remaut; Xavier Saelens

doi:10.1128/JVI.02105-15

Crystal Structure of the Conserved Amino Terminus of the Extracellular Domain of Matrix Protein 2 of Influenza A Virus Gripped by an Antibody

J Virol. 2015 Oct 14;90(1):611-5. doi: 10.1128/JVI.02105-15. Print 2016 Jan 1.

Authors

Ki Joon Cho¹, Bert Schepens¹, Kristof Moonens², Lei Deng¹, Walter Fiers¹, Han Remaut², Xavier Saelens³

Affiliations

¹ Medical Biotechnology Center, VIB, Ghent, Belgium Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
² Structural and Molecular Microbiology, Structural Biology Research Center, VIB, Brussels, Belgium Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium.
³ Medical Biotechnology Center, VIB, Ghent, Belgium Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium xavier.saelens@vib-ugent.be.

Abstract

We report the crystal structure of the M2 ectodomain (M2e) in complex with a monoclonal antibody that binds the amino terminus of M2. M2e extends into the antibody binding site to form an N-terminal β-turn near the bottom of the paratope. This M2e folding differs significantly from that of M2e in complex with an antibody that binds another part of M2e. This suggests that M2e can adopt at least two conformations that can elicit protective antibodies.

MeSH terms

Animals
Antibodies, Monoclonal / chemistry*
Antibodies, Monoclonal / isolation & purification
Antibodies, Monoclonal / metabolism
Antibodies, Viral / chemistry*
Antibodies, Viral / isolation & purification
Antibodies, Viral / metabolism
Cell Line
Crystallography, X-Ray
Humans
Mice, Inbred BALB C
Protein Binding
Protein Conformation
Viral Matrix Proteins / chemistry*
Viral Matrix Proteins / metabolism

Substances

Antibodies, Monoclonal
Antibodies, Viral
M2 protein, Influenza A virus
Viral Matrix Proteins

Associated data

PDB/4N8C

Grants and funding

Fonds Wetenschappelijk Onderzoek (FWO) provided postdoctoral fellowships to Bert Schepens and Kristof Moonens. The Hercules Foundation funded the structural biology infrastructure under grant UABR/09/005. Access to the Diamond Light Source was supported in part by EU FP7 infrastructure grant BIOSTRUCT-X (contract 6601).