Persisters: Methods for Isolation and Identifying Contributing Factors--A Review

Sarah E Rowe; Brian P Conlon; Iris Keren; Kim Lewis

doi:10.1007/978-1-4939-2854-5_2

Persisters: Methods for Isolation and Identifying Contributing Factors--A Review

Methods Mol Biol. 2016:1333:17-28. doi: 10.1007/978-1-4939-2854-5_2.

Authors

Sarah E Rowe¹, Brian P Conlon¹, Iris Keren¹, Kim Lewis²

Affiliations

¹ Antimicrobial Discovery Center, Department of Biology, Northeastern University, 134 Mugar Hall, 360 Huntington Ave., Boston, MA, 02115, USA.
² Antimicrobial Discovery Center, Department of Biology, Northeastern University, 134 Mugar Hall, 360 Huntington Ave., Boston, MA, 02115, USA. k.lewis@neu.edu.

PMID: 26468096
DOI: 10.1007/978-1-4939-2854-5_2

Abstract

Persister cells are phenotypic variants surviving a lethal dose of antibiotic, sufficient to kill the bulk of an exponential phase population. In this chapter we summarize current techniques to isolate persisters and discuss limitations associated with identifying mechanisms of persister formation.

Keywords: Antibiotic; Biofilm; Hip mutant; Toxin-antitoxin (TA) modules.

Publication types

Review

MeSH terms

Anti-Bacterial Agents / therapeutic use*
Biofilms / drug effects*
Biofilms / growth & development
Drug Resistance, Bacterial / genetics*
Escherichia coli / drug effects
Escherichia coli / genetics
Humans
Infections / drug therapy
Infections / genetics
Infections / microbiology*
Microbial Viability / drug effects

Substances

Anti-Bacterial Agents