Abstract
We studied the influence of fucoidans from brown algae Fucus evanescens, Laminaria cichorioides and Laminaria japonica on apoptosis of human peripheral blood lymphocytes. It was demonstrated that fucoidans induced lymphocyte apoptosis, increased the proportion of cells with low mitochondrial transmembrane potential, and inhibit expression of Bcl-xL gene in blood lymphocytes. These findings suggest that lymphocyte apoptosis induced by the analyzed sulfated polysaccharides is mediated by mitochondrial pathway.
Keywords:
apoptosis; fucoidan; mitochondrial membrane potential; periperal blood lymphocytes.
MeSH terms
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Apoptosis / drug effects
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Apoptosis / genetics
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Fucus / chemistry*
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Gene Expression / drug effects
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Humans
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Laminaria / chemistry*
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Leukocytes, Mononuclear / cytology
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Leukocytes, Mononuclear / drug effects*
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Leukocytes, Mononuclear / metabolism
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Membrane Potential, Mitochondrial / drug effects
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Mitochondria / drug effects
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Mitochondria / metabolism
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Phaeophyceae / chemistry*
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Polysaccharides / isolation & purification
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Polysaccharides / pharmacology*
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Primary Cell Culture
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Sulfates
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bcl-X Protein / antagonists & inhibitors*
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bcl-X Protein / genetics
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bcl-X Protein / metabolism
Substances
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BCL2L1 protein, human
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Polysaccharides
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Sulfates
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bcl-X Protein