Evaluation of a multiplex real-time polymerase chain reaction assay for the detection of influenza and respiratory syncytial viruses

Diagn Microbiol Infect Dis. 2016 Jan;84(1):40-42. doi: 10.1016/j.diagmicrobio.2015.09.014. Epub 2015 Sep 25.

Abstract

Nasopharyngeal swabs from 424 children were used to compare the performances of the new multiplex real-time polymerase chain reaction (RT-PCR) RIDA®GENE Flu & RSV kit and monospecific RT-PCR assays in detecting respiratory syncytial and influenza viruses. The easy-to-use kit was highly sensitive and specific and is recommended for routine practice.

Keywords: Influenza; PCR; RSV; RT-PCR; Respiratory viruses.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Child
  • Child, Preschool
  • Female
  • Humans
  • Infant
  • Influenza, Human / diagnosis*
  • Male
  • Molecular Diagnostic Techniques / methods*
  • Multiplex Polymerase Chain Reaction / methods*
  • Nasopharynx / virology
  • Orthomyxoviridae / genetics
  • Orthomyxoviridae / isolation & purification*
  • Real-Time Polymerase Chain Reaction / methods*
  • Respiratory Syncytial Virus Infections / diagnosis*
  • Respiratory Syncytial Viruses / genetics
  • Respiratory Syncytial Viruses / isolation & purification*
  • Sensitivity and Specificity