Zebrafish is a valuable model organism to study vertebrate development, organ regeneration and to generate human disease models. As an important member of the arsenal of genome editing, TALE nucleases (TALENs) have implicated in broad applications in zebrafish reverse genetic studies. In this chapter, we describe the detailed protocols of TALEN-mediated genome manipulations in zebrafish, including targeted gene disruption by indel mutations, deletion of large genomic regions by using two pairs of TALENs, and precise genome modification by homologous recombination (HR).
Keywords: Chromosomal deletion; Gene disruption; Gene targeting; Genome manipulation; Homologous recombination; Reverse genetics; TALE nuclease; TALEN; Targeted mutagenesis; Zebrafish.