Melanin Biosynthesis Inhibition Effects of Ginsenoside Rb2 Isolated from Panax ginseng Berry

J Microbiol Biotechnol. 2015 Dec 28;25(12):2011-5. doi: 10.4014/jmb.1505.05069.

Abstract

Ginsenoside Rb2 (Gin-Rb2) was purified from the fruit extract of Panax ginseng. Its chemical structure was measured by spectroscopic analysis, including HR-FAB-MS, (1)H-NMR, and IR spectroscopy. Gin-Rb2 decreased potent melanogenesis in melan-a cells, with 23.4% at 80 μM without cytotoxicity. Gin-Rb2 also decreased tyrosinase and MITF protein expression in melan-a cells. Furthermore, Gin-Rb2 presented inhibition of the body pigmentation in the zebrafish in vivo system and reduced melanin contents and tyrosinase activity. These results show that Gin-Rb2 isolated from P. ginseng may be an effective skin-whitening agent via the in vitro and in vivo systems.

Keywords: Panax ginseng; ginsenoside Rb2; melan-a cell; melanin biosysnthesis; zebrafish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Ginsenosides / chemistry
  • Ginsenosides / isolation & purification
  • Ginsenosides / metabolism*
  • Melanins / analysis
  • Melanins / antagonists & inhibitors*
  • Melanocytes / drug effects*
  • Monophenol Monooxygenase / analysis
  • Monophenol Monooxygenase / antagonists & inhibitors
  • Panax / chemistry*
  • Spectrum Analysis
  • Zebrafish

Substances

  • Ginsenosides
  • Melanins
  • ginsenoside Rb2
  • Monophenol Monooxygenase