Thyroid disrupting effects of polychlorinated biphenyls in ovariectomized rats: A benchmark dose analysis

Abstract

Polychlorinated biphenyls (PCBs) are proved endocrine disrupting potentials. Reference points (RP) for PCBs are derived from dose-response relationship analysis by using the traditional no observed adverse effect or lowest observed adverse effect level (NOAEL/LOAEL) methods, or a more advanced benchmark dose (BMD) method. In present study, toxicological RP for PCBs' thyroid disruption was established and compared between NOAEL/LOAEL and BMD method in an ovariectomized (OVX) rat model. Sham and OVX controls were given corn oil while other OVX groups were administered with 0.1, 1.0, 5.0, and 10.0mg/kg bw of PCBs (aroclor 1254) respectively by gavage. Body weight change, liver type I 5'-deiodinase (5'-DI) activity, serum total thyroxine (tT4), triiodothyroxine (tT3), thyroid stimulating hormones (TSH), and thyroid histopathological changes were measured and analyzed. In PCBs-treated groups, serum tT4, tT3, TSH, and histopathological examinations showed significant changes with a dose-dependent manner compared with those in OVX control (P<0.05). The toxicological RP for PCBs affecting thyroid function of OVX rats was 0.02 mg/kg'bw based on BMD analysis.

Keywords: Benchmark dose analysis; Polychlorinated biphenyls; Reference points; Thyroid disruption.