Eucalyptus granlla, which was one of dominant plantations in south China, was deemed as the important wood bio resources. However, the small molecules of Eucalyptus granlla wood weren't effectively reused. Thus the molecules of wood extractives in Eucalyptus granlla were extracted and studied so as to further utilize the resources. The result suggested that the optimal extraction time of ethanol/methanol extraction, petroleum ether/acetic ether extraction, and benzene/alcohol extraction were 5h, 7h and 4h, respectively. The wood extractives included hexanedioic acid, bis (2-ethylhexyl) ester, 3,3,7,11-tetramethyltricyclo[5.4.0.0(4,11)] undecan-1-ol, squalene, etc. and wood extractives of Eucalyptus granlla was suitable for extraction of 1,5-hexadien-3-yne and squalene.