Background: Primary culture of rat osteoblast cells serves as a model in in vitro research of bone metabolism. But most of studies about osteoblast use fetal or newborn rats for cell isolation, including researches about the post-menopausal osteoporosis. This will affect the authenticity and reliability of the results. These differences have been a concern in recent years. So far, little research has focused on adult female rat osteoblasts.
Methods: In this study, a systematic study was done regarding the proliferation, differentiation, and mineralization of adult female rat osteoblasts.
Results: The study showed that the age of the host may affect the growth and the mineralization ability of osteoblast.
Conclusions: The result of the study suggests that in vitro studies related to osteoblasts should select the right host and appropriate observation points according to the cell growth characteristics in order to ensure the consistency of the actual changes.