Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan

Polina A Kameneva; Kseniya V Efimova; Viacheslav G Rybin; Tatiana Y Orlova

doi:10.3390/toxins7103947

Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan

Toxins (Basel). 2015 Sep 28;7(10):3947-59. doi: 10.3390/toxins7103947.

Authors

Polina A Kameneva^{1

2}, Kseniya V Efimova^{3

4}, Viacheslav G Rybin⁵, Tatiana Y Orlova⁶

Affiliations

¹ Zhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo St., 17, Vladivostok 690041, Russia. kameneva.p.a@gmail.com.
² Far Eastern Federal University, Suhanova St., 8, Vladivostok 690950, Russia. kameneva.p.a@gmail.com.
³ Zhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo St., 17, Vladivostok 690041, Russia. xengen88@gmail.com.
⁴ Far Eastern Federal University, Suhanova St., 8, Vladivostok 690950, Russia. xengen88@gmail.com.
⁵ Zhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo St., 17, Vladivostok 690041, Russia. vgrybin@yahoo.com.
⁶ Zhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo St., 17, Vladivostok 690041, Russia. torlova06@mail.ru.

Abstract

For the first time the presence of dinophysistoxin-1 (DTX-1) in a culture of Prorocentrum foraminosum was revealed in cells and in the culture medium. The clone was isolated from coastal waters of the Sea of Japan and identified by molecular analyses of SSU and D1/D2 regions of LSU rDNA. The concentration of DTX-1 in cells was 8.4 ± 2.5 pg/cell and, in cell-free media, 27.9 ± 14.7 µg/L. The toxin presence was confirmed by HPLC with high-resolution tandem mass-spectrometry.

Keywords: DTX-1; HPLC-HRMS; Prorocentrum foraminosum; Russia; molecular identification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid
Clone Cells
Culture Media / chemistry
Dinoflagellida / cytology
Dinoflagellida / genetics
Dinoflagellida / metabolism*
Environmental Monitoring / methods*
Harmful Algal Bloom
Mass Spectrometry
Oceans and Seas
Okadaic Acid / analogs & derivatives
Pyrans / isolation & purification*
Russia
Seawater / analysis
Seawater / parasitology
Species Specificity

Substances

Culture Media
Pyrans
Okadaic Acid
dinophysistoxin 1