Spatiotemporally Controlled Reorganization of Signaling Complexes in the Plasma Membrane of Living Cells

Abstract

Triggered immobilization of proteins in the plasma membrane of living cells into functional micropatterns is established by using an adaptor protein, which is comprised of an antiGFP nanobody fused to the HaloTag protein. Efficient in situ reorganization of the type I interferon receptor subunits as well as intact, fully functional signaling complexes in living cells are achieved by this method.

Keywords: cell micropatterning; cytokine signaling; fluorescence imaging; imaging, plasma membranes, type I interferon receptors; microcontact printing.