Complete genome sequence of Clostridium pasteurianum NRRL B-598, a non-type strain producing butanol

Karel Sedlar; Jan Kolek; Helena Skutkova; Barbora Branska; Ivo Provaznik; Petra Patakova

doi:10.1016/j.jbiotec.2015.09.022

Complete genome sequence of Clostridium pasteurianum NRRL B-598, a non-type strain producing butanol

J Biotechnol. 2015 Nov 20:214:113-4. doi: 10.1016/j.jbiotec.2015.09.022. Epub 2015 Sep 26.

Authors

Karel Sedlar¹, Jan Kolek², Helena Skutkova³, Barbora Branska⁴, Ivo Provaznik⁵, Petra Patakova⁶

Affiliations

¹ Department of Biomedical Engineering, Brno University of Technology, Technicka 12, 616 00 Brno, Czech Republic. Electronic address: sedlar@feec.vutbr.cz.
² Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague, Czech Republic. Electronic address: jan.kolek@vscht.cz.
³ Department of Biomedical Engineering, Brno University of Technology, Technicka 12, 616 00 Brno, Czech Republic. Electronic address: skutkova@feec.vutbr.cz.
⁴ Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague, Czech Republic. Electronic address: barbora.branska@vscht.cz.
⁵ Department of Biomedical Engineering, Brno University of Technology, Technicka 12, 616 00 Brno, Czech Republic. Electronic address: provaznik@feec.vutbr.cz.
⁶ Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague, Czech Republic. Electronic address: petra.patakova@vscht.cz.

PMID: 26410453
DOI: 10.1016/j.jbiotec.2015.09.022

Abstract

The strain Clostridium pasteurianum NRRL B-598 is non-type, oxygen tolerant, spore-forming, mesophilic and heterofermentative strain with high hydrogen production and ability of acetone-butanol fermentation (ethanol production being negligible). Here, we present the annotated complete genome sequence of this bacterium, replacing the previous draft genome assembly. The genome consisting of a single circular 6,186,879 bp chromosome with no plasmid was determined using PacBio RSII and Roche 454 sequencing.

Keywords: Butanol; Clostridium pasteurianum; Genome; Oxygen tolerance; Pyrosequencing; Single molecule real-time sequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Butanols / metabolism*
Clostridium / genetics*
Clostridium / metabolism*
DNA, Bacterial / analysis
DNA, Bacterial / genetics
Genome, Bacterial / genetics*
Sequence Analysis, DNA

Substances

Butanols
DNA, Bacterial

Associated data

GENBANK/CP011966