Enrichment and identification of Δ(9)-Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants

Kerstin Lange; Ansgar Poetsch; Andreas Schmid; Mattijs K Julsing

doi:10.1016/j.dib.2015.07.033

Enrichment and identification of Δ(9)-Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants

Data Brief. 2015 Aug 6:4:641-9. doi: 10.1016/j.dib.2015.07.033. eCollection 2015 Sep.

Authors

Kerstin Lange¹, Ansgar Poetsch², Andreas Schmid³, Mattijs K Julsing⁴

Affiliations

¹ Laboratory of Chemical Biotechnology, Department of Biochemical & Chemical Engineering, TU Dortmund University, 44227 Dortmund, Germany ; Department of Solar Materials, Helmholtz Centre for Environmental Research (UFZ), 04318 Leipzig, Germany.
² Plant Biochemistry, Ruhr University Bochum, 44801 Bochum, Germany.
³ Department of Solar Materials, Helmholtz Centre for Environmental Research (UFZ), 04318 Leipzig, Germany.
⁴ Laboratory of Chemical Biotechnology, Department of Biochemical & Chemical Engineering, TU Dortmund University, 44227 Dortmund, Germany.

Abstract

This data article refers to the report Δ(9)-Tetrahydrocannabinolic acid synthase (THCAS) production in Pichia pastoris enables chemical synthesis of cannabinoids (Lange et. al. 2015) [2]. THCAS was produced on a 2 L lab scale using recombinant P. pastoris KM71 KE1. Enrichment of THCAS as a technically pure enzyme was realized using dialysis and cationic exchange chromatography. nLC-ESI-MS/MS analysis identified THCAS in different fractions obtained by cationic exchange chromatography.