Comparison of biomass and lipid production under ambient carbon dioxide vigorous aeration and 3% carbon dioxide condition among the lead candidate Chlorella strains screened by various photobioreactor scales

Naoko Kobayashi; Austin Barnes; Travis Jensen; Eric Noel; Gunjan Andlay; Julian N Rosenberg; Michael J Betenbaugh; Michael T Guarnieri; George A Oyler

doi:10.1016/j.biortech.2015.08.124

Comparison of biomass and lipid production under ambient carbon dioxide vigorous aeration and 3% carbon dioxide condition among the lead candidate Chlorella strains screened by various photobioreactor scales

Bioresour Technol. 2015 Dec:198:246-55. doi: 10.1016/j.biortech.2015.08.124. Epub 2015 Sep 1.

Authors

Naoko Kobayashi¹, Austin Barnes¹, Travis Jensen¹, Eric Noel², Gunjan Andlay³, Julian N Rosenberg⁴, Michael J Betenbaugh⁴, Michael T Guarnieri⁵, George A Oyler⁶

Affiliations

¹ Department of Biochemistry, University of Nebraska-Lincoln, 1901 Vine Street, Lincoln, NE 68588, United States.
² School of Biological Science, University of Nebraska-Lincoln, 1104 T Street, Lincoln, NE 68588, United States.
³ Synaptic Research, 1448 South Rolling Road, Baltimore, MD 21227, United States.
⁴ Department of Chemical & Biomolecular Engineering, John Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, United States.
⁵ National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401, United States.
⁶ Department of Biochemistry, University of Nebraska-Lincoln, 1901 Vine Street, Lincoln, NE 68588, United States; Department of Chemical & Biomolecular Engineering, John Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, United States; Synaptic Research, 1448 South Rolling Road, Baltimore, MD 21227, United States.

PMID: 26398668
DOI: 10.1016/j.biortech.2015.08.124

Abstract

Chlorella species from the UTEX collection, classified by rDNA-based phylogenetic analysis, were screened based on biomass and lipid production in different scales and modes of culture. The lead candidate strains of C. sorokiniana UTEX 1230 and C. vulgaris UTEX 395 and 259 were compared between conditions of vigorous aeration with filtered atmospheric air and 3% CO2 shake-flask cultivation. The biomass of UTEX 1230 produced 2 times higher at 652 mg L(-1) dry weight under both ambient CO2 vigorous aeration and 3% CO2 conditions, while UTEX 395 and 259 under 3% CO2 increased to 3 times higher at 863 mg L(-1) dry weight than ambient CO2 vigorous aeration. The triacylglycerol contents of UTEX 395 and 259 increased more than 30 times to 30% dry weight with 3% CO2, indicating that additional CO2 is essential for both biomass and lipid accumulation in UTEX 395 and 259.

Keywords: Biomass; Chlorella sorokiniana; Chlorella vulgaris; Lipid content; rDNA-based phylogenetic tree.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Biomass*
Carbon Dioxide / metabolism*
Chlorella / metabolism*
Lipids / biosynthesis*
Photobioreactors*
Triglycerides / metabolism

Substances

Lipids
Triglycerides
Carbon Dioxide